Allen, Richard (2021). CIL:34536, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QC02FD

High resolution view of the nuclear envelope of both the macronucleus, seen here, and the micronucleus consists of 2 membranes containing numerous perforations called pores. Proteins are imported through these pores and ribosomal subunits are transported out of the macronucleus through the same pores. TEM taken on 8/16/67 by R. Allen with a Philips 200 operating at 60kV. Neg. 12,400X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 8253

• Tetrahymena pyriformis

• Cell by organism
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Macronucleus
• Nuclear membrane
• Nuclear pore

• Macronucleus organization
• Nuclear membrane organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 34536

Doi: https://doi.org/10.6075/J0QC02FD

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL34536

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12