Lee, Chan-Soo; Choi, Chang-Ki; Shin, Eun-Young; Schwartz, Martin Alexander; Kim, Eung-Gook (2021). CIL:26526, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PG1QH8

To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, NIH3T3 cells were co-stained for βPIX (green) and myosin IIA (red). As a member of the Dbl family of Rho-GEFs (guanine nucleotide exchange factors), βPIX could contribute to cell motility through interactions with myosin. Findings from this study help elucidate a functional link between MII and Rac1/Cdc42 GTPases, which may regulate protrusion/adhesion dynamics in migrating cells. This image is original data from Fig. S2, "Colocalization of MII and GEFs in NIH3T3 fibroblasts," in J. Cell Biol. 2010. Vol. 190(4):663–674

• 2021

• Choi, Chang-Ki
• Kim, Eung-Gook
• Lee, Chan-Soo
• Schwartz, Martin Alexander
• Shin, Eun-Young

Cells were cultured in DME (Invitrogen) supplemented with 10% fetal bovine serum and 100 U/ml penicillin/streptomycin (Invitrogen) at 37°C in a humidified 5% CO2 incubator. Cells were fixed using 3.7% paraformaldehyde in PBS for 15 min, permeabilized using 0.2% Triton X-100 in PBS for 2 min, blocked with 2% BSA in PBS, and stained with the indicated primary antibodies for at 4°C overnight, followed by incubation with a secondary Alexa Fluor 488–, 546– or 594–conjugated antibody. Images were captured by Leica TCS SP2 confocal microscope with HCX PL APO 63X objective.

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Alexa Fluor 488; Alexa Fluor 546
Data qualification: Raw

• Cell Image Library Group ID: 8283

• Mus musculus

• Actin filament
• Fibroblast
• Lamellipodium
• Myosin II complex

• NIH/3T3
• Regulation of cell migration

• data
• image

• No linguistic content; Not applicable

Samplenumber: 26526

Doi: https://doi.org/10.6075/J0PG1QH8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL26526

Reference

• J. Cell Biol. 2010. Vol. 190(4):663–674. https://www.ncbi.nlm.nih.gov/pubmed/?term=0713598

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12