Microscopy product ID: 14
2D Image
Description | Zero tilt image from a single tilt series through a 2 ?m thick section of a medium spiny neuron that has been intracellularly injected and photooxidized |
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Description | Tar file containing full sized unaligned tilt images in Suprim format (tomo1.*.f) along with the fiducial mark file used to align them (tomo1.fido) and the aligned, cropped images used for the reconstruction (tomo1.*.crop). An animation of the aligned, cropped images may be viewed by clicking on the animation link. Note the basename for the 2D data files (tomo1) is different than the basename for the volume (acc1_2ma). |
Animation
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Description | Animation through the aligned tilt series |
Segmentation
Description | Manual segmentation of dendritic spines using Xvoxtrace 2.11 from tomographic volume of spiny dendrite from mouse nucleus accumbens. Each spine was segmented as a separate object. Spines were surfaced and rendered using Synu. The display image shows a rendering of the dendritic spines (multiple colors) and the dendritic shaft (gray). The dark blue face on the dendritic shaft represents the shorn face of the dendrite at the volume surface. |
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Description | Tar file containing the segmented spines and dendritic shaft from the tomographic volume. Tarfile contains objects surfaced with Synu (*.synu), the associated Viewdata file required by Synu for viewing; and Xvoxtrace file (acc1_2ma.trace) used to contour the objects. Clicking on "All details" link in the segmentation window will return a list of segmented objects and their descriptions. |
Reconstruction
Description | Tomograhic reconstruction of portion of spiny dendrite from a medium spiny neuron from mouse nucleus accumbens. Neuron was filled with Lucifer Yellow and then photooxidized. To view correlated light microscopic data, click View Correlated Data |
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Description | Tar file containing the tomographic volume in Analyze 7.5 format (acc1_2ma.img/hdr). |
Animation
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Description | maximum intensity projection through spiny dendrite rotated in 5 degrees increments on the y axis. Contrast is reversed so that the stained dendrite appears bright against a dark background |
Full metadata
- Collection
- Cite This Work
-
Martone, Maryann; Bushong, Eric A. (2017). Microscopy product ID: 14. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0KP81ZM
- Creation Date
- Microscopy product: 2002-04-19. Experiment: 2001-12-13. Project: 2001-09-01.
- Date Issued
- 2017
- Research Team Head
- Researcher
- Technical Details
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Male. Strain: C57BL/6
Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000 IVEM
- Funding
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NIH
- Series
- Scientific Name
- Anatomy
- Topic
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Related Resource
- Microscopy product 14 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB14
Other version
- License
-
Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
-
Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22