Withers, Ginger; Brandner, Dieter (2021). CIL:4617, Rattus, multipolar neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0862GH8

Dendritic development in cultured hippocampal neurons after 17 days in vitro. Neurons were immunostained for MAP2, a microtubule associated protein localized to dendrites but not axons to show the extent and complexity of the dendritic arbor at this relatively mature stage of development.

• 2021

• Brandner, Dieter
• Withers, Ginger

Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained MAP2 (HM2, from Sigma with d549 conjugated secondary, excitation, 555, emission, 568, Jackson Immunoresearch). Images were acquired with a Leica DMRA microscope with a 20X (Fluotar, NA 0.5) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software.

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw

• Cell Image Library Group ID: 9912

• Rattus

• Cytoskeleton
• Dendrite
• Microtubule cytoskeleton
• Multipolar neuron

• Dendrite development
• Neuron development

• data
• image

• No linguistic content; Not applicable

Samplenumber: 4617

Doi: https://doi.org/10.6075/J0862GH8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL4617

Creative Commons Attribution 4.0 International Public License

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2022-08-12