CIL:34603, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:34603, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0C827ZT
- Description
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A cross section of cilium through the cilium-basal body complex extending from the cilium to the transition zone between the cilium and basal body and ending at the proximal tip of the basal body. In cross section the 9 + 2 axoneme of the cilium stands out. The plasma membrane covers the axoneme and is continuous with the plasma membrane covering the cell body. Each doublet tilts inward to the tangent of the axonemal cylinder. Two arms, an inner arm and outer arm, extend from the complete microtubule of each doublet. These arms have ATPase activity and are called dyneins. They cause one doublet to slide against the adjacent doublet. Other protein links, like the spokes, in the axoneme are elastic and induce the characteristic bending of the cilium and prevent excess sliding as the doublets slide past each other. TEM taken on 7/15/67 by R. Allen with RCA EMU3F operating at 50kV. Neg. 19,200X. Adapted with permission. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.
- Date Issued
- 2021
- Researcher
- Methods
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Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements - Series
- Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 34603
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL34603
- J. Cell Biol. 40:716-733, 1969 https://www.ncbi.nlm.nih.gov/pubmed/?term=5765762
Source data
Reference
- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12