Davis, Margaret I. (2021). CIL:16245, Mus musculus, CNS neuron (sensu Vertebrata), interneuron, hippocampal granule cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0BP0208

Distribution of interneurons expressing EGFP from the GAD65 promoter in the day 14 mouse hippocampus colabelled for the CB1 cannabinoid receptor (red) and counterstained with DAPI (blue) to show the cell layers. EGFP expression was amplified with chicken anti-GFP (Abcam, 1:2000). GAD65-EGFP expressing neurons are enriched in the dentate gyrus and a band of EGFP is present in the inner dentate molecular layer. CB1 immunoreactivity (L15 rabbit polyclonal 1:200, K. Mackie) is prominent in the molecular layer but also present at lower levers in the outer portion of the dentate molecular layer. Diffuse CB1 immunoreactivity is also present in the hilus. Section (40 um) was mounted with Fluoromount G and imaged with a 10X, 0.3 NA objective on a Zeiss Axiovert microscope. AF568 was excited at 562/40 nm and emission collected at 624/40 nm with Semrock filters and a single band dichroic (530-585 nm reflected, 601-800 nm transmitted). AF488 was excited at 482/35 nm and emission collected at 536/40 nm with Semrock filters and a single band dichroic (446-499 nm reflected, 513-735 nm transmitted). DAPI was excited at 350/50 nm and emission collected at 460/50 nm with a CLP400 dichroic (Chroma). Single images were captured for each channel using a Zeiss Axiocam MR CCD and pseudo-colored using Axiovision. Linear background subtraction was used on the presented image.

• 2021

• Davis, Margaret I.

Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: fluorescence microscopy
Imaging mode: recorded image
Parameter imaged: fluorescence emission
Source of contrast: compartmentalization of stain or label; distribution of a specific protein
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); Alexa Fluor 488; Alexa Fluor 568
Processing history: unprocessed raw data

• Mus musculus

• Axon
• CNS neuron (sensu Vertebrata)
• Hippocampal granule cell
• Interneuron
• Neuronal cell body membrane
• Nucleus

• Cannabinoid receptor activity
• Cell communication by chemical coupling

• data
• image

• No linguistic content; Not applicable

Samplenumber: 16245

Doi: https://doi.org/10.6075/J0BP0208

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL16245

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12