Ball, David; Peccoud, Jean (2021). CIL:35718, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01G0MCZ

A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged NET1.

NET1 is a core subunit of the RENT complex that involves in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure.

These phase,gfp images are part of an image group that ranges from CIL:35700-35719. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

• 2021

• Ball, David
• Peccoud, Jean

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions

• Cell Image Library Group ID: 10629

• Saccharomyces cerevisiae

• NET1 protein
• Nucleolus
• Nucleus
• RENT complex

• Cell cycle
• Cell division
• Chromatin silencing at rDNA
• Mitosis
• Nucleolus organization
• RDNA binding
• Regulation of exit from mitosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35718

Doi: https://doi.org/10.6075/J01G0MCZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35718

Reference

• Ball DA, Marchand J, Poulet M, Baumann WT, Chen KC, Tyson JJ, Peccoud J. Oscillatory dynamics of cell cycle proteins in single yeast cells analyzed by imaging cytometry. PLoS One. 2011;6(10):e26272. https://doi.org/10.1371/journal.pone.0026272
• PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26. https://www.ncbi.nlm.nih.gov/pubmed/?term=22046265

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2022-08-12