Ris, Hans (2021). CIL:35382, Homo sapiens, cevical carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09022RP

Whole human female metaphase chromosome from metaphase arrested cell swollen in hypotonic medium and treated to remove histones. The resulting residual chromosome 'scaffold' was imaged at 1 MeV. This image was taken with a specimen tilt of 45 degrees. Grouped with it is an image of the same area at 55 degrees tilt, providing an oblique stereo view of the chromosome.

• 2021

• Ris, Hans

HeLa cells were metaphase-arrested by a 3hr treatment with colcemid, the cells mechanically broken by passage through a 22-gauge needle, exposed to hypotonic 75 mM KCl for 10 min. Samples were fixed with 3:1 methanol:acetic, treated to remove histones, spread on a water surface, picked up on formvar coated grids, critical point dried, and imaged with the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic microscopy of chromosomes. Meth Cell Biol 22:77-96.

Preparation: coagulating-fixative fixed tissue; critical_point dried specimen
Relation to intact cell: whole mounted tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8650

• Homo sapiens

• Cevical carcinoma
• Condensed chromosome

• HeLa
• Nuclear division

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35382

Doi: https://doi.org/10.6075/J09022RP

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35382

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2022-08-12