Allen, Richard (2021). CIL:13105, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0DB80Z6

High resolution image of the cross section through a radial arm of the contractile vacuole complex. The collecting canal occupies the center of the arm whose membrane is supported by the radial microtubular ribbon which has now divided into at least six segments and surrounds the canal. Gaps between the segments are where the mass of membrane tubules of the smooth spongiome opens into the canal. Decorated tubules occurring in bundles form the peripheral layer of the radial arm. TEM taken on 5/2/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 18,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 3941

• Paramecium multimicronucleatum

• Cell by organism
• Ciliated Protist
• Contractile vacuole
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule associated complex
• Radial canal of the contractile vacuole complex

• Contractile vacuole organization
• Microtubule-based process
• Water transport

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13105

Doi: https://doi.org/10.6075/J0DB80Z6

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13105

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12