Xu, Z.; Ogawa, H.; Vagnarelli, P.; Bergmann, J.H.; Hudson, D.F.; Ruchard, S.; Fukagawa, T.; Earnshaw, W.C.; Samejima, K. (2021). CIL:12159, Gallus gallus gallus, memory B cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0B27T18

Cell of the DT40 chicken B lymphocyte line at metaphase. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is a central z-slice. Localization of exogenous TrAP-INCENP (red) plus endogenous Aurora B(green) in INCENP-OFF cells expressing TrAP-INCENP-F802A. Chromosomes stained with DAPI (blue). With this INCENP mutation, INCENP and Aurora B are co-localized. See Fig 4G panel 1 in Xu et al. 2009 J Cell Biol 187, 637-653.

• 2021

• Bergmann, J.H.
• Earnshaw, W.C.
• Fukagawa, T.
• Hudson, D.F.
• Ogawa, H.
• Ruchard, S.
• Samejima, K.
• Vagnarelli, P.
• Xu, Z.

Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed

• Cell Image Library Group ID: 3878

• Gallus gallus gallus

• Chromosome, centromeric region
• Memory B cell
• Nuclear chromosome
• Spindle

• DT40
• Mitosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12159

Doi: https://doi.org/10.6075/J0B27T18

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12159

Reference

• JCB 178:1207-1221, 2007 https://www.ncbi.nlm.nih.gov/pubmed/?term=17893245
• Yam PT, Wilson CA, Ji L, Hebert B, Barnhart EL, Dye NA, Wiseman PW, Danuser G, Theriot JA. Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. J Cell Biol. 2007 Sep 24;178(7):1207-21. https://doi.org/10.1083/jcb.200706012

Other resource

• BioStudies (previously JCB DataViewer) [search for content released in 2014]: https://www.ebi.ac.uk/biostudies/JCB/studies?#

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2022-08-12