Civelekoglu-Scholey, Gul; Tao, Li; Brust-Mascher, Ingrid; Wollman, Roy; Scholey, Jonathan M. (2021). CIL:30566, Drosophila melanogaster, early embryonic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J05H7F40

A confocal 4D-stack from a transgenic Drosophila embryo expressing lamin-GFP (green) and injected with rhodamine-conjugated tubulin (red) and anti-lamin-B antibodies during prometaphase. The microinjection of a mixture of anti-lamin-B mAbs crosslinks the lamin-B envelope into a hyperstable network that fails to disassemble and remains throughout mitosis. This structure impedes spindle elongation. This image is original data contributing to Fig. 6 "Functional perturbation of the lamin-B envelope interferes with spindle length changes and the completion of mitosis" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68.

• 2021

• Brust-Mascher, Ingrid
• Civelekoglu-Scholey, Gul
• Scholey, Jonathan M.
• Tao, Li
• Wollman, Roy

Embryos expressing lamin-B-GFP were collected at 25°C for 1 h, matured for 40 min, dechorionated, placed on heptane glue and covered with halocarbon oil. For injections, embryos were dehydrated for 3-6 min, covered with halocarbon oil and injected (as described in Brust-Mascher & Scholey, 2009). For double injections, embryos were allowed to recover for 5–20 min between injections. Images from this image group were acquired with an inverted IX-70 Olympus with Ultra-View spinning disk confocal head (PerkinElmer) and acquired with an oil immersion objective (UPlan-Apochromat 100x N.A. 1.35, or Plan-Apochromat 60x NA 1.4). Time series (at intervals of 3 to 10 sec) z-stacks of planes at 0.5 µm were acquired with an Orca II CCD camera (Hamamatsu Photonics). For further information see J. Cell Biol. 188(1):49-68.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Green fluorescent proteins from Aequorea; Rhodamine
Data qualification: Raw

• Cell Image Library Group ID: 8578

• Drosophila melanogaster

• Early embryonic cell
• Kinesin complex
• Nuclear lamina
• Spindle microtubule

• Regulation of microtubule-based process
• Regulation of mitotic prometaphase

• data
• image

• No linguistic content; Not applicable

Samplenumber: 30566

Doi: https://doi.org/10.6075/J05H7F40

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL30566

Reference

• J Cell Biol. 2010 Jan 11;188(1):49-68. https://doi.org/10.1083/jcb.200908150

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12