Allen, Richard (2021). CIL:36738, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0W9584M

Cells taken from stationary phase cultures often have larger lysosomes which contain additional paracrystalline layers. These lysosomes may be on their way to becoming the crystals which appear in larger numbers in stationary phase cells. TEM taken on 12/31/70 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 44,125X. Bar = 0.1µm.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Paramecium multimicronucleatum

• Cell by organism
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Lysosome
• Primary lysosome

• Digestive system process

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36738

Doi: https://doi.org/10.6075/J0W9584M

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36738

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2022-08-12