Valerio-Santiago, Mauricio; Monje-Casas, Fernando (2021). CIL:13896, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01G0K0M

DIC image of cells constitutively expressing Tem1 localized to both spindle pole bodies (SPBs) and expressing Kin4 from the GAL1-10 promoter growing in medium with galactose. Constitutive loading of Tem1 to SPBs allowed cells to progress through the cell cycle despite Kin4 overexpression. Compare to control image CIL# 13897. Image is Fig 5C bottom right panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.

• 2021

• Monje-Casas, Fernando
• Valerio-Santiago, Mauricio

Cells (MATa tem1::KanMX ura3::eGFP-CNM67–TEM1::URA3 kin4::TRP1-pGAL1-10-KIN4) were grown in rich medium containing 2% galactose/ 2% raffinose. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (leica) and ImageJ software.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 9034

• Saccharomyces cerevisiae

• Spindle pole body

• ATP binding
• Cell division
• GTPase activity
• Mitosis
• Mitotic cell cycle spindle orientation checkpoint
• Nucleotide binding
• Protein binding
• Protein phosphorylation
• Protein serine/threonine kinase activity
• Regulation of exit from mitosis
• Small GTPase mediated signal transduction
• W303

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13896

Doi: https://doi.org/10.6075/J01G0K0M

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13896

Reference

• J Cell Biol. (2011) 192: 599-614. https://www.ncbi.nlm.nih.gov/pubmed/?term=21321099

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12