Murray, John; Hu, Ke (2021). CIL:10557, Toxoplasma gondii RH. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GT5M9F

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged Tgalpha1tubulin and mRFP-tagged TgDLC (dynein light chain). Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X, 1.4 NA. Filters: EGFP, ex 450-490 em 500-540, mRFP, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. Raw data stack corresponds to deconvolved data stack: CIL# 10558 and DIC image: CIL# 10569.

Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

• 2021

• Hu, Ke
• Murray, John

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Yellow fluorescent proteins from Aequorea; mRFP1
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 5588

• Toxoplasma gondii RH

• Centriole
• Conoid
• Cortical microtubule
• Dynein complex
• Spindle pole

• ATPase activity, coupled
• Microtubule cytoskeleton organization
• Microtubule-based movement
• Motor activity
• Regulation of cell shape
• Structural constituent of cytoskeleton
• Toxoplasma gondii RH stable cell line
• Toxoplasmosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10557

Doi: https://doi.org/10.6075/J0GT5M9F

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10557

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2022-08-12