CIL:36229, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:36229, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0H41Q2F
- Description
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A composite of cross sections through the cilium-basal body complex extending from the cilium to the transition zone between the cilium and basal body and ending at the proximal tip of the basal body. Bars = 0.2µm. a. Cross section of cilium. TEM taken 8/4/65 by R. Allen with RCA EMU3F operating at 50kV. b. Cross section near the proximal end of the cilium. Triangular densities link the doublets to the membrane. TEM taken 7/23/65 by R. Allen with RCA EMU3F operating at 50kV. c. Level of singlet ends and narrowing of plasma membrane around the axoneme. TEM Taken 8/7/65 by R. Allen with RCA EMU3F operating at 50kV. d. Similar to c showing the curving of the plasma membrane and the enclosed alveoli. TEM taken 8/7/65 by R. Allen with RCA EMU3F operating at 50kV. e. Distal end of basal body showing 9 triplets and the electron opaque central core. The coated end of a parasomal sac lies anterior to the basal body. TEM taken 8/9/65 by R. Allen with RCA EMU3F operating at 50kV. f. Toward the proximal end of the basal body. TEM taken on 8/6/65 by R. Allen with RCA TEM. g. Proximal ends of two basal bodies showing the cartwheel end in one and just distal to the cartwheel in the second basal body. kd, kinetodesmal fiber; pc mt, postciliary microtubules. TEM taken 8/7/65 by R. Allen with RCA TEM. Published in J. Cell Biol. 40:716-733, 1969. Adapted with permission. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Date Issued
- 2021
- Researcher
- Methods
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Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed - Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 36229
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36229
- J Cell Biol. 1969 Mar;40(3):716-33. https://www.ncbi.nlm.nih.gov/pubmed/?term=5765762
Source data
Reference
- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12