Buchanan, JoAnn (2021). CIL:6518, Danio rerio, Outer plexiform layer of retina, Bipolar cell of retina. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00R9NH1

This study of the zebrafish eye is a series of four images taken at increasing magnifications. The first image is the SEM block face of the zebrafish eye. This is followed by a second image that is a higher magnification TEM image of the plexiform layer and the outer nuclear layer of the zebrafish retina. This image, the third image in the series, is a 15,000X TEM image of the bipolar and horizontal cells interdigitating in the OPL layer of the zebrafish retina. The fourth image in the series is a higher magnification (60,000X) of the dense synaptic bar and synaptic vesicles.

Bipolar and horizontal cells (H) interdigitate in the outer plixiform layer (OPL) of the zebrafish retina. The presynaptic cone membrane contains an arciform density (arrowhead) and synaptic vesicles (V). Image collected on a JOEL 1230 at 80 kV using a Gatan 967 slow-scan, cooled CCD camera at 15,000X. 70 nm thick section.

Microwave processing, osmium tetroxoxide/osmid procedure for enhancing membranes, and copper lead en bloc staining.
Procedure for specimen preparation available at http://www.stanford.edu/~redhair/JoAnn_Buchanan.

• 2021

• Buchanan, JoAnn

Preparation: osmium tetroxide fixed tissue; glutaraldehyde fixed tissue; heat fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide; lead salt; copper salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 1769

• Danio rerio

• Bipolar cell of retina
• Outer plexiform layer of retina
• Presynaptic membrane
• Retina
• Retinal outer plexiform layer
• Ribbon synapse
• Synapse

• Horizontal cell of retina
• Retina layer formation
• Synaptic transmission

• data
• image

• No linguistic content; Not applicable

Samplenumber: 6518

Doi: https://doi.org/10.6075/J00R9NH1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL6518

Reference

• JoAnn Buchanan: https://alleninstitute.org/what-we-do/brain-science/about/team/staff-profiles/joann-buchanan/
• Related resource: http://www.stanford.edu/~redhair/JoAnn_Buchanan/Zebrafish_Retina.html

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12