von Dassow, George; Verbrugghe, Koen J.C.; Miller, Ann L.; Sider, Jenny R.; Bement, William M. (2021). CIL:13400, Xenopus laevis laevis. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FF3R1N

A normal X. laevis blastomere expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). Single microtubules extend to the cortex, even in these large cells. X. laevis embryos were imaged on a laser scanning confocal microscope (1024; Bio-Rad Laboratories) mounted on an Axiovert microscope (Carl Zeiss, Inc.) using a 63× 1.4 NA Plan-Apochromat oil objective. Video corresponds to Supplementary Figure 1D and Video 2 from J Cell Biol. 2009 Dec 14;187(6):831-45.

• 2021

• Bement, William M.
• Miller, Ann L.
• Sider, Jenny R.
• Verbrugghe, Koen J.C.
• Von Dassow, George

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 6184

• Xenopus laevis laevis

• Cell cortex
• Ensconsin
• Microtubule

• Embryo
• Microtubule polymerization

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 13400

Doi: https://doi.org/10.6075/J0FF3R1N

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13400

Reference

• J Cell Biol. 2009 Dec 14;187(6):831-45. https://www.ncbi.nlm.nih.gov/pubmed/?term=20008563

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12