Maddox, Paul S.; Straight, Aaron; Coughlin, Peg; Mitchison, Timothy J.; Salmon, Edward D. (2021). CIL:13089, Xenopus [NCBITaxon:262014]. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J03F4NDM

Dual-channel spinning-disk confocal time-lapse movie showing kinetochores (Alexa488 labeled CENP-A antibody) in metaphase (red) relative to poleward microtubule flux. Fluorescent speckle microscopy (FSM) of microtubules and kinetochore imaging during metaphase was performed as described in Maddox et al. (2002, 2003), and with a 100X/1.4 NA Plan Apochromat objective (Nikon) with 2X2 binning in the cooled CCD camera (model ER; Hamamatsu Corporation). MetaMorph® software (Universal Imaging Corp.) was used to control shutters, wavelength selection, image acquisition, and storage. Images were acquired at 5-s intervals. Sequential images of different fluorophores were acquired at 3-15s intervals, depending on the experiment. Paired images from different color channels were within 0.5 s of each other. Movie is video 2 from J Cell Biol 2003 162:377-382.

• 2021

• Coughlin, Peg
• Maddox, Paul S.
• Mitchison, Timothy J.
• Salmon, Edward D.
• Straight, Aaron

Preparation: unfixed tissue
Relation to intact cell: cell extract
Item type: recorded image
Imaging mode: spinning disk confocal microscopy; fluorescent speckle microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine; Alexa Fluor 488; labeled primary antibody
Processing history: color combine
Data qualification: Raw;intensitiesquantitation

• Cell Image Library Group ID: 3925

• Xenopus

• Kinetochore
• Spindle microtubule

• Mitotic metaphase
• Poleward microtubule flux

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13089

Doi: https://doi.org/10.6075/J03F4NDM

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13089

Reference

• J Cell Biol 2003 162:377-382 https://www.ncbi.nlm.nih.gov/pubmed/?term=12900391

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2022-08-12