Cammer, Michael; Novikoff, Phyllis (2021). CIL:35479, Homo sapiens, hepatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0G73CPV

Fluorescence image of tripolar mitosis in a liver cancer cell line. Normally, dividing cells form a single metaphase plate of paired chromosomes which are pulled apart by opposing microtubule spindles. This Z series obtained using a laser scanning confocal microscope shows abnormal division by three sets of spindles.
This image group includes other z-series and a 3D surface representation.

• 1993-12-01

• 2021

• Cammer, Michael
• Novikoff, Phyllis

HuH-7 cells are an immortalized cultured cell line derived from hepatic carcinoma. Cells were grown on 1.5 glass coverslips, fixed with formaldehyde and glutareldehyde, extracted, and labeled for fluorescence microscopy with commercial antibodies to alpha- or beta-tubulin and propidium iodide. The coverslip was mounted on a slide in PBS and glycerol with n-propyl gallate as an antifading agent. Imaging was performed with a BioRad MRC 600 laser scanning confocal microscope with a Kr/Ar laser with lines at 488 and 568 nm. The microscope was a fixed tube length Nikon Diaphot with 60X N.A. 1.4 phase 3 optics. Z series was collected at 0.2 um step sizes. The original XY spatial scale has been lost.

Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Fluorescein (FITC); propidium iodide
Processing history: unprocessed raw data
Data qualification: Processed

• Cell Image Library Group ID: 11176

• Homo sapiens

• Hepatocyte
• Liver
• Spindle microtubule

• Chromosome organization
• Chromosome segregation
• Cytokinesis
• HuH-7
• Mitotic metaphase
• Sclerosing hepatic carcinoma

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35479

Doi: https://doi.org/10.6075/J0G73CPV

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35479

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2023-08-17