Allen, Richard (2021). CIL:36778, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PG1QMM

The three ciliary membranelles in the buccal cavity from which this class of ciliates gets its name, Oligohymenophorea, are each composed of four rows of basal body/cilium complexes. The quadrulus has four rows of cilia less tightly packed and interspersed with parasomal sacs. The dorsal and ventral peniculi are more tightly packed. In this micrograph only 6 or 7 rows of peniculi are present at this level since the rows of the peniculi do not go all the way to the posterior end but rows end in a staggered way somewhere along the length of the buccal cavity. The quadrulus borders the cytopharynx over its full length and goes to the posterior end of the buccal cavity. TEM taken on 3/30/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,750X. Bar = 0.5µm.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39162) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 10069

• Paramecium caudatum

• Cell by organism
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Oral apparatus

• Oral apparatus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36778

Doi: https://doi.org/10.6075/J0PG1QMM

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36778

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12