Gelfand, Vladimir; Larsen, Adam (2021). CIL:40629, Drosophila melanogaster. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0736PR2

Fluorescence microscopy time series of Drosophila S2 cell stably expressing mCherry-alpha-tubulin and spreading on a ConA-coated substrate in the presence of cyto D. Long processes containing microtubule bundles are formed. Microtubule looping and entry into the bundles appears to contribute to the growth of the processes.
This image stack is part of a grouped set that includes a corresponding time lapse movie.

• 2021

• Gelfand, Vladimir
• Larsen, Adam

Time series of widefield fluorescence images were recorded using a Nikon U-2000 inverted microscope equipped with a Nikon 100x NA 1.49 objective lens, Nikon perfect Focus system and Cascade II EMCCD camera (Roper Scientific)driven by Metamorph software. See also Fig 3C and Supplemental Movie S8 in A.L. Jolly et al. 2010 Proc Natl Acad Sci USA 107:12151-12156.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: electron multiplying CCD (EMCCD)
Imaging mode: widefield illumination; fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: mCherryFP

• Cell Image Library Group ID: 11370

• Drosophila melanogaster

• Cytoskeleton
• Microtubule

• Cytoskeleton organization
• S2

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40629

Doi: https://doi.org/10.6075/J0736PR2

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40629

Reference

• A.L. Jolly et al. 2010 Proc Natl Acad Sci USA 107:12151-12156. https://www.ncbi.nlm.nih.gov/pubmed/?term=20566873
• Morris E Feldman,Beth Apsel,Aino Uotila,Robbie Loewith,Zachary A Knight,Davide Ruggero,Kevan M Shokat (2009). Active-Site Inhibitors of mTOR Target Rapamycin-Resistant Outputs of mTORC1 and mTORC2. https://doi.org/10.1371/journal.pbio.1000038

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2022-08-12