Bowman, Jeff S.; Connors, Beth (2021). Raw flow cytometry data collected at the Ellen Browning Scripps Pier, Volume 1. In Scripps Ecological Observatory. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0ZG6SD4

Sea surface samples are collected twice weekly in collaboration with the Scripps Pier Shore Station component of the Southern California Coastal Ocean Observing System (SCCOOS). Samples for flow cytometry are fixed to 0.25 % with glutaraldehyde, pre-filtered through a 60 micron mesh, and run with and without SYBR Green on a Guava 11HT flow cytometer. Fisher 123count eBeads are used as an internal standard. The expected concentration of beads is 5000 per mL.

• 2019-10-28 to present

• 2021

• Bowman, Jeff S.

• Connors, Beth
• Scripps Ecological Observatory

Simons Foundation Early Career Investigator in Marine Microbial Ecology and Evolution program.

• Scripps Ecological Observatory - Raw flow cytometry data

• Ellen Browning Scripps Memorial Pier (San Diego, Calif.)

• Bacteria
• Flow cytometry
• Phytoplankton

Point: 32.867103, -117.257357

• data
• image

• English

Identifier: Beth Connors: https://orcid.org/0000-0002-4992-4218

Identifier: Jeff S. Bowman: https://orcid.org/0000-0002-8811-6280

Doi: https://doi.org/10.6075/J0ZG6SD4

Reference

• Sample flow cytometry scripts (GitHub.com): https://github.com/bowmanlab/flow_cytometry_scripts

Other resource

• Scripps Ecological Observatory: https://ecoobs.ucsd.edu/

Creative Commons Attribution 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2023-06-06