Antipa, Gregory (2021). CIL:25901, uncultured scuticociliate, Conchophthirus curtus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09P30JF

This section of Conchophthirus is at the edge of the cortex showing the striated fibers and microtubules that attach to ciliated basal bodies. In particular, enlarged glancing longitudinal views of both the striated fibers and microtubules reveal features of their organization. This particular micrograph of the group shows the relationship between a striated fiber and microtubules with a basal body. The negative magnification is 30,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.

• 2021

• Antipa, Gregory

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 11100

• Conchophthirus curtus
• Uncultured scuticociliate

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cortical membranes
• Cortical microtubule
• Dense fibrillar component
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule
• Plasma membrane

• Cortical cytoskeleton organization
• Cortical microtubule organization
• Fibril organization
• Microtubule cytoskeleton organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 25901

Doi: https://doi.org/10.6075/J09P30JF

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25901

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12