Ris, Hans (2021). CIL:25514, Drosophila melanogaster, salivary gland. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0125RK2

Whole mount squash of polytene chromosome from Drosophila salivary gland. Image showing a series of bands and interbands was taken with a High Voltage EM at a tilt angle of 45 degrees. The accompanying grouped image, taken at 55 degrees forms a stereo pair, providing an oblique 3D view of the chromosome.

• 2021

• Ris, Hans

Salivary glands from 3rd instar larvae of Drosophila melanogaster were dissected, fixed with formalin-acetic acid containing 0.1% Triton-X 100, and squashed on a glass slide. After freezing in liquid nitrogen, the cover slips removed, and the slide thawed and stained with 1% uranyl acetate, rinsed in 50% ethanol, dehydrated and transferred to amyl acetate, and covered with 2% parlodion in amyl acetate. Selected areas of the parlodion film, are transferred to formvar coated EM grids, and the parlodion removed by immersion in amyl acetate (see Ris H, 1981 Stereoscopic Electron Microscopy of Chromosomes, Meth Cell Biol 22:77-96). Grids were observed using the Madison High Voltage Electron Microscope at 1 MeV.

Preparation: formaldehyde fixed tissue; acetic acid treatment
Relation to intact cell: squashed preparation
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8367

• Drosophila melanogaster

• Polytene chromosome
• Salivary gland

• data
• image

• No linguistic content; Not applicable

Samplenumber: 25514

Doi: https://doi.org/10.6075/J0125RK2

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25514

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2025-01-30