Tani, Tomomi; Allen, Richard (2021). CIL:10411, Paramecium multimicronucleatum, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01Z43KS

DVD quality video of the Contractile Vacuole Complex of Paramecium multimicronucleatum. In vivo CVC filling/expulsion cycles. A CV in a compressed cell is observed in side view undergoing cycles of filling (diastole) and expulsion (systole) of its fluid content as the CV membrane fuses then separates from the plasma membrane and with one radial arm. The radial arm fuses with the CV after systole then just before systole it again separates from the CV. As the CV empties fluid will flow into the end of the radial arm next to the CV that is called the ampullus. This fluid comes from the collecting canal of the same radial arm. There are actually 5 to 10 radial arms connected to one CV but the others are out of the focus plane or out of the field of focus. Movies were taken by Dr. Tomomi Tani with differential interference contrast optics attached to a Leica DMIRB inverted microscope. A Leica Fluotar x63 objective lens (NA = 0.7) was used.

• 2021

• Allen, Richard
• Tani, Tomomi

Preparation: living tissue
Relation to intact cell: compressed living tissue
Item type: microscopy; modes of light microscopy
Imaging mode: differential interference contrast microscopy
Parameter imaged: refractive index
Source of contrast: boundaries between regions with different refractive index
Visualization methods: miscellaneous fill
Processing history: film; unprocessed raw data

• Cell Image Library Group ID: 2665

• Paramecium multimicronucleatum

• Ciliated Protist
• Contractile vacuole
• Eukaryotic cell
• Eukaryotic Protist

• Cellular water homeostasis

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 10411

Doi: https://doi.org/10.6075/J01Z43KS

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10411

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=10607534
• Tani T, Allen RD, Naitoh Y. Periodic tension development in the membrane of the in vitro contractile vacuole of Paramecium multimicronucleatum: modification by bisection, fusion and suction. J Exp Biol. 2000 Jan;203(Pt 2):239-51. https://doi.org/10.1242/jeb.203.2.239

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12