Matsuda, A.; Shao, L.; Boulanger, J.; Kervrann, C.; Carlton, P.M.; Kner, P.; Agard, D.; Sedat, J. (2021). CIL:39746, Drosophila melanogaster, early embryonic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QJ7GKJ

PALM images of two regions of Drosophila metaphase chromosomes with red arrowheads pointing to filamentous structures. Overview images of the chromosome clusters are seen the the grouped image (CIL:39747)
See Fig 3 in: A Matsuda et al. (2010) Condensed mitotic chromosme structure at nanometer resolution using PALM and EGFP-histones. PLOSone 5:e12768.

• 2021

• Agard, D.
• Boulanger, J.
• Carlton, P.M.
• Kervrann, C.
• Kner, P.
• Matsuda, A.
• Sedat, J.
• Shao, L.

PALM was achieved using a custom wide-field microscope platform with 100x NA 1.4 objective lens or 150X TIRF NA 1.45 lens, yielding a pixel size at the CCD camera of 0.0792 or 0.0528 micrometers respectively.
See also: A Matsuda et al. (2010) Condensed mitotic chromosme structure at nanometer resolution using PALM and EGFP-histones. PLOSone 5:e12768.

Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: portrayed image
Imaging mode: PALM (photoactivation localization microscopy)
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: PALM reconstruction
Data qualification: Processed

• Cell Image Library Group ID: 10721

• Drosophila melanogaster

• Chromatin
• Early embryonic cell
• Nuclear chromosome

• Chromatin organization
• DNA packaging

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39746

Doi: https://doi.org/10.6075/J0QJ7GKJ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39746

Reference

• A Matsuda et al. (2010) Condensed mitotic chromosme structure at nanometer resolution using PALM and EGFP-histones. PLOSone 5:e12768 https://www.ncbi.nlm.nih.gov/pubmed/?term=20856676
• Atsushi Matsuda, Lin Shao, Jerome Boulanger, Charles Kervrann, Peter M. Carlton, Peter Kner, David Agard, John W. Sedat (2010). Condensed Mitotic Chromosome Structure at Nanometer Resolution Using PALM and EGFP- Histones. PLoS One. https://doi.org/10.1371/journal.pone.0012768

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2022-08-12