CIL:35167, Rattus rattus, podocyte (sensu Diptera), epithelial cell
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- Collection
- Cite This Work
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Cammer, Michael; Novikoff, Phyllis (2021). CIL:35167, Rattus rattus, podocyte (sensu Diptera), epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0K936GQ
- Description
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Dominant features of a nephron in the cortex of rat kidney showing f-actin localization in the glomerulus, a blood vessel, and proximal tubules. The glomerulus is the large ball at the left. The blood vessel that delivers blood to the glomerulus (Afferent arteriole) is the bright narrow tube shown both in elongated view and in cross section (upper right) that is lined with juxtaglomerular cells. The proximal convoluted tubules are cut to reveal the actin rich micro villi lining the interior surface.
This thin optical section was acquired with a laser scanning confocal microscope and is a montage of a few adjacent fields imaged at high resolution. - Creation Date
- 1994-12-01
- Date Issued
- 2021
- Researchers
- Methods
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Formaldehyde fixed rat kidney was cut by Vibratome and incubated with fluorescent labeled phalloidin, a toxin which binds to f-actin filaments. Imaging was performed using a BioRad MRC 600 laser scanning confocal microscope with a Kr/Ar laser. The microscope was a Nikon Diaphot with a 60X or 40X fixed tube length objective. Images of adjacent fields were manually stitched using Adobe Photoshop 2.5 on a Quadra Macintosh computer and may have manual contrast curves applied. Imaging performed at the Image Analysis Facility at the Albert Einstein College of Medicine.
- Technical Details
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Preparation: chemically fixed tissue; vibratome-sectioned tissue
Relation to intact cell: chemically fixed tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy; fluorescence microscopy
Parameter imaged: distribution of a specific protein
Source of contrast: fluorescence emission
Visualization methods: visualization of contiguous regions; fluorescent labels
Processing history: montage
Data qualification: Processed;spatialmeasurements - Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 35167
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35167
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-08-17