D'Amico, Leonard A.; Cooper, Mark S. (2021). CIL:11826, Danio rerio, yolk cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0P55M7V

Visualization of yolk syncytial layer (YSL) nuclei in living zebrafish embryos. Sytox Green, a membrane-impermiant vital nuclear stain, was injected into the yold cell of late blastula (sphere stage) embryos. At 36 hrs of development, the zebrafish yolk cell displays a prominent anterior-posterior (A, P) axis, with brightly displayed fluorescent nuclei distributed through its entire cortex.

• 2021

• Cooper, Mark S.
• D'Amico, Leonard A.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy; single-spot confocal microscopy
Parameter imaged: optical path length gradient; fluorescence emission
Source of contrast: boundaries between regions with different refractive index; differences in adsorption or binding of stain
Visualization methods: SYTOX Green
Processing history: unprocessed raw data
Data qualification: Raw;intensitiesquantitation

• Cell Image Library Group ID: 10026

• Danio rerio

• Yolk cell

• Anterior/posterior axis specification

• data
• image

• No linguistic content; Not applicable

Samplenumber: 11826

Doi: https://doi.org/10.6075/J0P55M7V

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11826

Reference

• D'Amico LA, Cooper MS. Morphogenetic domains in the yolk syncytial layer of axiating zebrafish embryos. Dev Dyn. 2001 Dec;222(4):611-24. https://doi.org/10.1002/dvdy.1216
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=11748830

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2022-08-12