Allen, Richard (2021). CIL:9808, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PC31G8

Opercularia coarctata. Transmission electron micrograph of contracted cell body. This ciliate does not have rows of basal body/cilia over its whole surface but has cilia in the oral region and in a trochal band area. The pellicle is sculpted into ridges and grooves that circle the body. A layer of endoplasmic reticulum lies under the pellicle next to a layer of myonemal fibers. The macronucleus, contractile vacuole, mitochondria, and peristomeare evident in this view. TEM taken on 6/12/69 by R. Allen with Philips 300 operating at 60kV. Neg. 4,270X. Bar = 1µm.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. A print of the negative was scanned and processed in Photoshop. This image is suitable for qualitative analysis. There is a high resolution version of this image in the library (CIL:39193) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 10120

• Opercularia
• Opercularia coarctata

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cytoplasm
• Eukaryotic cell
• Eukaryotic Protist
• Macronucleus
• Mitochondrion
• Oral apparatus

• Cambridge Culture Collection, Cambridge, England, # LB1657/1
• Cortical cytoskeleton organization
• Cytoplasm organization
• Macronucleus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9808

Doi: https://doi.org/10.6075/J0PC31G8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9808

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12