CIL:28783, Caenorhabditis elegans, embryo
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- Collection
- Cite This Work
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Maddox, Paul S.; Hyndman, Francie; Monen, Joost; Oegema, Karen; Desai, Arshad (2021). CIL:28783, Caenorhabditis elegans, embryo. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TB15JZ
- Description
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Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 (strain OD31) in which KNL-1 has been depleted by RNAi. Discrete foci of GFP signal within the nucleus coalesce along curvilinear chromosomes during prophase. After nuclear envelope breakdown, chromosomes fail to congress to the metaphase plate, but GFP-KNL-2 localization is evident. As seen after CeCENP-A depletion and CeCENP-C depletion (CIL 28779 and CIL 28782, respectively), foci of GFP-KNL-2 formed along the spindle after anaphase onset. The video is ∼11 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals (see Materials and methods), and corresponding data are shown in Fig. 3 in J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5.
- Date Issued
- 2021
- Researchers
- Methods
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Images were acquired via a spinning disk confocal microscope (CSU10; McBain Instruments) mounted on an inverted microscope (TE2000e; Nikon). Strain TH32 coexpressing GFP-histone H2b and GFP–γ-tubulin was imaged using a 60× 1.4 NA plan Apo objective with 1.5× auxiliary magnification and a cooled CCD camera (Orca ER; Hamamatsu) binning 2 × 2. Strain OD31 expressing GFP–KNL-2 was imaged in the same manner without the 1.5× auxiliary magnification.
- Technical Details
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Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data - Series
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 28783
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL28783
- J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5. https://www.ncbi.nlm.nih.gov/pubmed/?term=17339379
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- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12