Allen, Richard (2021). CIL:26265, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01V5CRP

When the antigen was visualized with immunogold using DS-1 on thin-frozen sections the antigen could be seen to be present on the periphery of the decorated tubules. The collecting canal and smooth spongiome were not labeled with this mAb. Please see the paper for details. TEM taken on 3/22/86 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5 µm. Adapted with permission from J. Cell Sci. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are outlined in Methods Cell Biol. 2010;96:143-73. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:1312) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 7025

• Paramecium multimicronucleatum

• Cell by organism
• Ciliated Protist
• Contractile vacuolar membrane
• Contractile vacuole
• Eukaryotic cell
• Eukaryotic Protist

• Contractile vacuole organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 26265

Doi: https://doi.org/10.6075/J01V5CRP

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL26265

Reference

• J. Cell Sci. 108:3163-3170, 1995 https://www.ncbi.nlm.nih.gov/pubmed/?term=7593277

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2022-08-12