Deshmukh, M.; Dimitru, R.; Gama, V.; Fagan, B.M.; Bower, J.J.; Swahari, V.; Pevney, L.H. (2021). CIL:42451, Homo sapiens, embryonic stem cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0BV7FZR

Immunofluorescence image of Human Embryonic Stem cells stained with an antibody to the active form of Bax (red) and Hoechst (blue). The distribution of Bax, an apoptosis mediator indicates that it resides in the Golgi apparatus under these conditions.

• 2021

• Bower, J.J.
• Deshmukh, M.
• Dimitru, R.
• Fagan, B.M.
• Gama, V.
• Pevney, L.H.
• Swahari, V.

Cells were fixed with formaldehyde, permeabilized using Triton X-100, and stained with anti-Bax antibody 6A7 which is specific to the active form (red) and Hoechst dye to stain nuclei (red). Images were recorded on a Hamamatsu ORCA-ER digital camera mounted on a Leica DMIRE2 inverted microscope.
See also:
Dumitru R, Gama V, Fagan BM, Bower JJ, Swahari V, Pevny LH, Deshmukh M. 2012. Human embryonic stem cells have constitutively active Bax at the Golgi and are primed to undergo rapid apoptosis. Mol Cell. 46(5):573-83.

Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization methods: Hoechst 33258; Alexa Fluor 488
Data qualification: Processed

• Homo sapiens

• Embryonic stem cell
• Golgi apparatus
• Nucleus

• Apoptosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 42451

Doi: https://doi.org/10.6075/J0BV7FZR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL42451

Other resource

• 2008 Olympus BioScapes Digital Imaging Competition®: https://www.olympus-lifescience.com/en/bioscapes/years/

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2022-08-12