CIL:42451, Homo sapiens, embryonic stem cell
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- Collection
- Cite This Work
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Deshmukh, M.; Dimitru, R.; Gama, V.; Fagan, B.M.; Bower, J.J.; Swahari, V.; Pevney, L.H. (2021). CIL:42451, Homo sapiens, embryonic stem cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0BV7FZR
- Description
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Immunofluorescence image of Human Embryonic Stem cells stained with an antibody to the active form of Bax (red) and Hoechst (blue). The distribution of Bax, an apoptosis mediator indicates that it resides in the Golgi apparatus under these conditions.
- Date Issued
- 2021
- Researchers
- Methods
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Cells were fixed with formaldehyde, permeabilized using Triton X-100, and stained with anti-Bax antibody 6A7 which is specific to the active form (red) and Hoechst dye to stain nuclei (red). Images were recorded on a Hamamatsu ORCA-ER digital camera mounted on a Leica DMIRE2 inverted microscope.
See also:
Dumitru R, Gama V, Fagan BM, Bower JJ, Swahari V, Pevny LH, Deshmukh M. 2012. Human embryonic stem cells have constitutively active Bax at the Golgi and are primed to undergo rapid apoptosis. Mol Cell. 46(5):573-83. - Technical Details
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Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization methods: Hoechst 33258; Alexa Fluor 488
Data qualification: Processed - Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 42451
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL42451
- 2008 Olympus BioScapes Digital Imaging Competition®: https://www.olympus-lifescience.com/en/bioscapes/years/
Source data
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- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12