CIL:38867, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Cite This Work
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Allen, Richard (2021). CIL:38867, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0W0958K
- Description
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Euplotes sp. (eurystomus ?) is a hypotrich. This ciliate has a few rows of dorsal cilia as well as specialized clumps of cilia called cirri on its ventral side and a complex of membranelles involved in feeding called the adoral zone of membranelles. The cell is flattened dorso-ventrally. This electron micrograph is a longitudinal section dividing dorsal from ventral parts of the cell and exposing the oral cavity, the thread-like macronucleus, the extended contractile vacuole, contractile vacuole pore, and a large assortment of food vacuoles many of which contained material that was lost during sectioning leaving holes in the section. The cytopharynx and nascent food vacuole along with the discoidal (pharyngeal) vesicles are also seen. TEM taken on 8/3/67 by R. Allen with Philips 200 operating at 60kV. Neg. 1,370X. Bar = 5µm. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
- Date Issued
- 2021
- Researcher
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation - Series
- Scientific Name
- Anatomy
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- No linguistic content; Not applicable
- Identifier
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Samplenumber: 38867
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL38867
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12