Hartman, Tiffiney R.; Zinshteyn, Daniel; Schofield, Heather K.; Nicolas, Emmanuelle; Okada, Ami; O'Reilly, Alana M. (2021). CIL:13750, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RV0MF5

In the absence of boi (boi[e] mutant), Hedgehog (Hh, red) is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13744, 13745). Expression of a mutant Boi that lacks the Ptc-binding domain in apical cells relocalizes Hh to apical cells (this image; boi[e]; UAS-boi[∆FN2]/+; babGal4/+). Image is Fig 4G in J Cell Biol. 2010. 191: 943-952.

• 2021

• Hartman, Tiffiney R.
• Nicolas, Emmanuelle
• O'Reilly, Alana M.
• Okada, Ami
• Schofield, Heather K.
• Zinshteyn, Daniel

Fly ovaries were dissected and fixed as described previously (O'Reilly et al., 2008). For anti-Boi immunostaining, ovaries were fixed in 2% formaldehyde on ice. Primary antibodies were 1:50 rat anti-Boi (Hartman et al. 2010), 1:100 goat anti-Hh (Santa Cruz Biotechnology, Inc.). Secondary antibodies used were FITC, Cy3, and/or Cy5 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe).

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 8382

• Drosophila melanogaster

• Cytoplasmic membrane-bounded vesicle
• Endocytic vesicle
• Extracellular region
• Follicle cell
• Follicle stem cell
• Germ line cell
• Germ line stem cell

• Boi[e]
• Cell adhesion
• Cysteine-type endopeptidase activity
• Germ-line stem cell division
• Morphogen activity
• Ovarian follicle cell development
• Patched binding
• Positive regulation of hh target transcription factor activity
• Positive regulation of smoothened signaling pathway
• Protein binding
• Smoothened signaling pathway

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13750

Doi: https://doi.org/10.6075/J0RV0MF5

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13750

Reference

• J Cell Biol. 2010. 191: 943-952. https://www.ncbi.nlm.nih.gov/pubmed/?term=21098113

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12