Korobova, Farida; Svitkina, Tatyana (2021). CIL:40664, Rattus, hippocampal neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0JH3JZW

Platinum replica illustrating the cytoskeletal organization of dendritic spines from extracted 14 DIV neurons. Thin spines associate with dendrites at the base (bottom) and with axons by the head (top). Unlabeled insets show the smaller versions of the corresponding main panels with axons color-coded in purple, dendrites in yellow, and spines in cyan. Thick fibers in neurites represent microtubules. Yellow boxes are enlarged in panels with corresponding numbers. Box 4, interaction of actin filaments (cyan) with an axonal microtubule (red). Box 5, branched actin filaments (cyan) in the neck of respective spines.This image is part of a group of images representing Figure 2B and its components from Mol Biol Cell. 2010 Jan 1;21(1):165-76. Figure 2B (this image) appears as CIL 40664. The uncolorized overview panel is CIL 40667. Box 4 appears as CIL 40665, and Box 5 appears as CIL 40666.

• 2021

• Korobova, Farida
• Svitkina, Tatyana

Please refer to referenced article for details on cell extraction and processing for electron microscopy.

Preparation: detergent permeabilized; glutaraldehyde fixed tissue; critical_point dried specimen
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: colorized

• Cell Image Library Group ID: 11503

• Rattus

• Actin cytoskeleton
• Dendritic shaft
• Dendritic spine
• Hippocampal neuron
• Microtubule cytoskeleton

• Dendrite morphogenesis
• Dendritic spine organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40664

Doi: https://doi.org/10.6075/J0JH3JZW

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40664

Reference

• Korobova F, Svitkina T. Molecular architecture of synaptic actin cytoskeleton in hippocampal neurons reveals a mechanism of dendritic spine morphogenesis. Mol Biol Cell. 2010 Jan 1;21(1):165-76. https://doi.org/10.1091/mbc.e09-07-0596
• Mol Biol Cell. 2010 Jan 1;21(1):165-76. Epub 2009 Nov 4. https://www.ncbi.nlm.nih.gov/pubmed/?term=19889835

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2022-08-12