CIL:39127, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:39127, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00V8C5W
- Description
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Ciliates such as Paramecium have specialized sites for endocytosis and exocytosis. Most of their surface is not available for internalization of membrane because of the underlying alveolar sac system. However to the right of each basal body or pair of basal bodies is an indentation of the plasma membrane called the parasomal sac. This penetrates through the septum and ends as a coated pit. This micrograph also illustrates the normal view of mitochondria in P. caudatum which are filled with tubular cristae. In cacodylate-buffered fixations the cytosol contains abundant electron-opaque glycogen storage granules which seem to be removed during collidine-buffered fixations. TEM taken on 5/28/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X.
- Date Issued
- 2021
- Researcher
- Methods
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Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements - Series
- Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 39127
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39127
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12