von Dassow, George; Verbrugghe, Koen J.C.; Miller, Ann L.; Sider, Jenny R.; Bement, William M. (2021). CIL:15794, Dendraster excentricus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0S75F1Q

Single-plane sequences of anucleate, centrosome-containing cytoplasts (sand dollars) expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). This anucleate cytoplast has four centrosomes, two in focus. In normal cells this size, furrowing would begin between 03:00 and 07:00, but no furrow occurs even 20 min later. Centrosomes are closer than the distance to the equator. Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C. Video corresponds to the first segment of video 6 and Fig 7A in J Cell Biol. 2009 Dec 14;187(6):831-45.

• 2021

• Bement, William M.
• Miller, Ann L.
• Sider, Jenny R.
• Verbrugghe, Koen J.C.
• Von Dassow, George

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 6184

• Dendraster excentricus

• Centrosome
• Ensconsin
• Microtubule

• Anucleate cytoplast
• Mitotic anaphase

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 15794

Doi: https://doi.org/10.6075/J0S75F1Q

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL15794

Reference

• J Cell Biol. 2009 Dec 14;187(6):831-45. https://www.ncbi.nlm.nih.gov/pubmed/?term=20008563

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2022-08-12