Allen, Richard (2021). CIL:12080, Paramecium tetraurelia, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0Z31XRK

High resolution image of microtubules that sometimes extend deep into the cytosol from the basal bodies. They serve to guide the trichocysts to their docking sites at the plasma membrane (see Plattner, Westphal and Tiggemann, J. Cell Biol. 92:368-377, 1982). Immunogold labeled for anti-b-tubulin detects the location of microtubules. TEM taken on 7/5/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 7,900X.

• 2021

• Allen, Richard

Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The raw film was scanned with a Nikon Coolscan 9000ED. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 9632

• Paramecium tetraurelia

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cortical microtubule
• Cortical microtubule cytoskeleton
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule basal body
• Trichocyst

• Cortical microtubule organization
• Microtubule basal body organization
• Microtubule cytoskeleton organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12080

Doi: https://doi.org/10.6075/J0Z31XRK

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12080

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12