CIL:12576, Mus musculus
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- Collection
- Cite This Work
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Zhou, Chao; Jenkins, Michael W.; Garita, Barbara; Han, Mingda; VanAuker, Michael; Rollins, Andrew M.; Watanabe, Michiko; Fujimoto, James G.; Linask, Kersti K. (2021). CIL:12576, Mus musculus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0NV9H0K
- Description
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A wild-type mouse embryos on embryonic day 9.5 was isolated, fixed in 3.5% para-formaldehyde in PBS, rinsed in PBS, and was imaged with a prototype optical coherence microscopy (OCM) imaging system. With OCM an optical beam is directed at the tissue, and a small portion of this light that reflects from sub-surface features is collected. Note that most light is not reflected but, rather, scatters. Scattered light can be filtered out using optical coherence. Only the reflected (non-scattered) light is coherent (i.e., retains the optical phase that causes light rays to propagate in one or another direction). In the OCM, an optical interferometer is used to detect only coherent light. The OCM data was acquired over a 400um x 400um field of view at 2 frames per second. The spacing between each 3D-OCM slice is 5um along the depth dimension. The OCM data on the mouse embryo confirmed that myocardial-endocardial radial associations exist in the intact ED 9.5 looping mouse heart.
- Date Issued
- 2021
- Researchers
- Technical Details
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Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: optical coherence microscope
Parameter imaged: reflected light
Source of contrast: interferometric coherence
Visualization methods: inherent scattering
Processing history: unprocessed raw data - Scientific Name
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 12576
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12576
Source data
- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12