Verma, Inder; Friedmann-Morvinski, Dinorah; Bushong, Eric; Ellisman, Mark (2021). CIL:47059, Mus musculus castaneus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0BC3X4F

Glioblastoma multiforme (GBM) is the most common and aggressive malignant primary brain tumor in humans. Here we show that gliomas can originate from differentiated cells in the central nervous system (CNS), including cortical neurons. Transduction by oncogenic lentiviral vectors of neural stem cells (NSCs), astrocytes, or even mature neurons in the brains of mice can give rise to malignant gliomas. All the tumors, irrespective of the site of lentiviral vector injection (the initiating population), shared common features of high expression of stem or progenitor markers and low expression of differentiation markers. Microarray analysis revealed that tumors of astrocytic and neuronal origin match the mesenchymal GBM subtype. We propose that most differentiated cells in the CNS upon defined genetic alterations undergo dedifferentiation to generate a NSC or progenitor state to initiate and maintain the tumor progression, as well as to give rise to the heterogeneous populations observed in malignant gliomas.

• 2021

• Bushong, Eric
• Ellisman, Mark
• Friedmann-Morvinski, Dinorah
• Verma, Inder

Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; compartmentalization of stain or label
Visualization methods: fluorescent labels; 4',6-diamidino-2-phenylindole (DAPI)

• Cell Image Library Group ID: 16854

• Mus musculus castaneus

• data
• image

• No linguistic content; Not applicable

Samplenumber: 47059

Doi: https://doi.org/10.6075/J0BC3X4F

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL47059

Previous version

• Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0BC3ZC0

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2022-08-12