Hu, Chi-Kuo (2021). CIL:7245, Homo sapiens, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0X92980

HeLa cell in cytokinesis was fixed and stained for microtubules (green), RacGAP1 (red), and DNA (blue).

Cells were fixed with MeOH on ice for 3 min, and stained with a primary antibody against RacGAP1 (Rac GTPase-activating protein 1), TOPRO3 (Invitrogen) for DNA, and FITC-DM1a antibody against microtubules. Secondary antibody was Alexa 594 for RacGAP1. A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective. Images were collected with an Orca ER CCD camera.
Lasers and filters: Innova 70C Spectrum 3 watt Laser, 488 Laser/ATOF 525/50, 568 Laser/ATOF 605/52, and 647 Laser ATOF 700/75.

• 2021

• Hu, Chi-Kuo

Preparation: methanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: labeled primary antibody; primary antibody plus labeled secondary antibody; membrane impermeant probe; Fluorescein (FITC); Alexa Fluor 594
Processing history: unprocessed raw data

• Cell Image Library Group ID: 1455

• Homo sapiens

• Epithelial cell
• Microtubule
• Nucleus

• Cytokinesis
• HeLa
• Rac GTPase binding

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7245

Doi: https://doi.org/10.6075/J0X92980

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7245

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2025-01-30