Ris, Hans (2021). CIL:7504, gallus gallus, erythrocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FJ2FTW

A drop of a preparation of chicken erythrocyte nuclei was exposed to low ionic strength and centrifuged onto a formvar-carbon film, fixed with paraformaldehyde and negatively stained with sodium phosphotungstate. Images were recorded at 40KX and accelerating voltage of 1MeV at the University of Wisconsin HVEM facility.This image was taken with a specimen tilt of 45 degrees. A grouped image of the same field at a 55 degree tilt provides an oblique stereo view of the tangle of chromatin fibers in the dispersed nucleus.

• 2021

• Ris, Hans

Preparation: glutaraldehyde fixed tissue
Relation to intact cell: spread nucleus
Item type: film
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: negative staining; tungsten compound
Processing history: unprocessed raw data

• Cell Image Library Group ID: 2460

• Gallus gallus

• Erythrocyte
• Nuclear chromatin

• DNA packaging

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7504

Doi: https://doi.org/10.6075/J0FJ2FTW

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7504

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=7022124
• Ris H. Stereoscopic electron microscopy of chromosomes. Methods Cell Biol. 1981;22:77-96. https://doi.org/10.1016/s0091-679x(08)61871-3

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2022-08-12