Brunet, Stephane; Dumont, Julien; Lee, Karen W.; Kinoshita, Kazuhisha; Hikal, Pascale; Gruss, Oliver J.; Maro, Bernard; Verlhac, Marie-Helene (2021). CIL:35423, Mus musculus, oocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GF0TGG

Time-lapse microscopy of a b-Tubulin GFP expressing mouse oocyte injected with siCo-5h, a control siRNA. Images were taken every 20 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner.
Video S3. Found at: doi:10.1371/journal.pone.0003338.s003

• 2021

• Brunet, Stephane
• Dumont, Julien
• Gruss, Oliver J.
• Hikal, Pascale
• Kinoshita, Kazuhisha
• Lee, Karen W.
• Maro, Bernard
• Verlhac, Marie-Helene

Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Green fluorescent proteins from Aequorea; visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw

• Cell Image Library Group ID: 8819

• Mus musculus

• Microtubule
• Microtubule organizing center part
• Nuclear chromosome
• Oocyte

• GTPase activity
• Meiotic spindle organization
• Microtubule organizing center organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35423

Doi: https://doi.org/10.6075/J0GF0TGG

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35423

Reference

• Brunet S, Dumont J, Lee KW, Kinoshita K, Hikal P, Gruss OJ, Maro B, Verlhac MH. Meiotic regulation of TPX2 protein levels governs cell cycle progression in mouse oocytes. PLoS One. 2008 Oct 3;3(10):e3338. https://doi.org/10.1371/journal.pone.0003338
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=18833336

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2022-08-12