Murray, John (2021). CIL:10479, Toxoplasma gondii RH. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MG7NMF

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged Tgbeta3-tubulin. Images overlaid with DIC. Images acquired using API DeltaVision on an Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: GFP, ex 450-490 em 500-540. Captured with CoolSnapHQ/ICX285. 3D stack is raw data corresponding to deconvolved stack: CIL# 10480.

Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

• 2021

• Murray, John

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD)
Imaging mode: fluorescence microscopy; differential interference contrast microscopy
Parameter imaged: fluorescence emission; optical path length gradient
Source of contrast: distribution of a specific protein; boundaries between regions with different refractive index
Visualization methods: EGFP
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 7515

• Toxoplasma gondii RH

• Cortical microtubule

• Microtubule cytoskeleton organization
• Regulation of cell shape
• Structural constituent of cytoskeleton
• Toxoplasma gondii RH stable cell line
• Toxoplasmosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10479

Doi: https://doi.org/10.6075/J0MG7NMF

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10479

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12