Ris, Hans (2021). CIL:35388, Drosophila melanogaster, salivary gland. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J03J3BVC

High Voltage (1MeV) TEM image of a 'thick' section of polytene chromosome from salivary gland of 3rd instar larva of Drosophila melanogaster showing the band-interband organization. This image was captured with a specimen tilt of 56 degrees. Grouped with it is an image of the same region taken at a tilt angle of 45 degrees, providing an oblique stereo view of the structure.

• 2021

• Ris, Hans

Salivary glands were dissected, fixed with paraformaldehyde, post-fixed with osmium tetroxide, and embedded in plastic. 4 micron sections were cut, stained with aqueous uranyl magnesium acetate, and observed with the Wisconsin HVEM at 1 MeV. See Ris, H 1981 Stereoscopic Electron Microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246

Preparation: chemically fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment; paraformaldehyde fixation
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11163

• Drosophila melanogaster

• Polytene chromosome
• Salivary gland

• Chromosome organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35388

Doi: https://doi.org/10.6075/J03J3BVC

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35388

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2022-08-12