Allen, Richard (2021). CIL:9696, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02Z14PQ

An image image of the multiple membrane systems at the cortex of ciliates. Thickened alveolar densities partially coat the alveolar membrane within the alveoli which underlie the plasma membrane of ciliates. The densities are seen particularly along the inner membrane at its corners with the septa. Fibrous systems lie within the cytoplasm next to these densities. Cross sections of four cilia are also seen. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/28/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:9017) is available for quantitative analysis.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print of negative scanned for Photoshop.
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 3221

• Coleps hirtus

• Alveolar membranes
• Axonemal dynein complex
• Axonemal microtubule
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Motile primary cilium
• Plasma membrane

• Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
• Ciliary or flagellar motility
• Microtubule cytoskeleton organization
• Multiple cell membranes
• Multiple membrane functions
• Structural molecule activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9696

Doi: https://doi.org/10.6075/J02Z14PQ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9696

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12