Wittmann, Torsten; Bokoch, Gary M.; Waterman-Storer, Clare M. (2021). CIL:11836, Potorous tridactylus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0C53JMD

Microtubules in a constitutively active Rac1(Q61L)-expressing PtK1 cell. Microtubules are subjected to continuous retrograde flow away from the cell edge, but many plus ends undergo net growth and remain close to the edge. The cell was microinjected with X-rhodamine-conjugated tubulin and an EGFP-Rac1(Q61L) expression plasmid, and was observed by fluorescence microscopy. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 2 of associated paper (J Cell Biol, 161:845-851, 2003).

• 2021

• Bokoch, Gary M.
• Waterman-Storer, Clare M.
• Wittmann, Torsten

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine
Processing history: unprocessed raw data
Data qualification: Raw;intensitiesquantitation

• Cell Image Library Group ID: 4105

• Potorous tridactylus

• Epithelial cell
• Microtubule

• Active Rac
• Microtubule polymerization
• Pt K1 (NBL-3)

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 11836

Doi: https://doi.org/10.6075/J0C53JMD

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11836

Reference

• J Cell Biol, 161:845-851, 2003. https://www.ncbi.nlm.nih.gov/pubmed/?term=12796474
• Wittmann T, Bokoch GM, Waterman-Storer CM. Regulation of leading edge microtubule and actin dynamics downstream of Rac1. J Cell Biol. 2003 Jun 9;161(5):845-51. https://doi.org/10.1083/jcb.200303082

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2022-08-12