CIL:40257, Rattus norvegicus, peripheral neuron
CIL source metadata (JSON)
File Size |
|
File Format |
|
- Collection
- Cite This Work
-
Ellisman, Mark H.; Perkins, Guy A. (2021). CIL:40257, Rattus norvegicus, peripheral neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RJ4JM0
- Description
-
A single image from animation of the computed slices through a tomographic volume of mitochondria at the Node of Ranvier in rat peripheral nerve root. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information, see: Perkins et al. Mitochondrial configurations in peripheral nerve suggest differential ATP production. J. Struct. Biol. (2010), doi:10.1016/j.jsb.2010.06.017 http://www.ncbi.nlm.nih.gov/pubmed/20600951
- Date Issued
- 2021
- Researchers
- Methods
-
Chemical fixation with 2% paraformaldehyde and 2.5% glutaraldehyde via vascular perfusion in 0.15M cacodylate buffer (pH 7.4) at 37 degrees C
Neuronal sections cut to a thickness of 300-500 nm were taken on 100 mesh copper clamshell grids. The sections on the grid were stained for 30 minutes to 60 minutes in 2% aqueous uranyl acetate then approximately 15 min in lead salts. 20nm fiducial markers were placed on the specimen. - Technical Details
-
Preparation: osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Parameter imaged: electron density
Data qualification: Processed - Series
- Scientific Name
- Anatomy
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Identifier
-
Samplenumber: 40257
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40257
- Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0V987WW
Source data
Previous version
- License
-
Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
-
Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
-
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12