Allen, Richard (2021). CIL:10005, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0CC0ZVF

Didinium nasutum. A tangential view of the surface of a non-dividing cell shows several ribbons of microtubules between the alveolar sac and the epiplasm. The thick fibrous layer associated with the epiplasm is evident, and the layer of mitochondria under the epiplasm is wrapped with rough ER. A peroxisome lies nearby and mucocysts and toxicysts are evident. TEM taken on 2/18/69 by R. Allen with Philips 300 operating at 60kV. Neg. 20,500X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:9928) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned to Photoshop.
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 3382

• Didinium nasutum

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cortical microtubule cytoskeleton
• Eukaryotic cell
• Eukaryotic Protist
• Extrusomes

• Carolina Biological Supply Company, NC, U.S.A.
• Cortical cytoskeleton organization
• Cortical microtubule organization
• Plasma membrane organization
• Structural constituent of cytoskeleton
• Structural molecule activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10005

Doi: https://doi.org/10.6075/J0CC0ZVF

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10005

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12