Ma, Hong; Samarabandu, Jagath; Devdhar, Recandu; Acharya, Raj; Cheng, Ping-chin; Meng, Chunling; Berezney, Ronald (2021). CIL:39235, Macropus sp. MG002, kidney cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GF0S51

Shown is a chromosome spread of a PtK1 cell pulsed with BrdU in early S phase, then treated with colcemid to arrest cells at metaphase. Spreads were immuno-labeled with FITC-conjugated anti-BrdU antibodies and counterstained with propidium iodide (red). Replication is confined to discrete sites of replication, some of which appear to occur in bands (arrows).

• 2021

• Acharya, Raj
• Berezney, Ronald
• Cheng, Ping-chin
• Devdhar, Recandu
• Ma, Hong
• Meng, Chunling
• Samarabandu, Jagath

Metaphase-arrested PtK1 Cells were swollen, pre-fixed in methanol-acetic acid, dropped onto cover glasses, incubated with H2O to promote spreading, and air dried. After hardening by a 4 hr incubation at 65C samples were fixed with 70% ethanol at -20C. Spreads were denatured with 4N HCl for 30 min at room temperature, and processed for immunofluorescence using an anti-BrdU antibody and FITC-conjugated secondary antibody. Cells were examined in a MRC-1024 confocal microscope mounted on a Nikon Optphot 2 microscope with 60x 1.4 NA objective lens.

See: Fig 6 in, Ma, H et al. 1998 Spatial and temporal dynamics of DNA replication sites in mammalian cells. J Cell Biol 143:1415-1425.

Preparation: ethanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: photomultiplier tube (PMT)
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Fluorescein (FITC); TexasRed
Processing history: montage
Data qualification: Processed

• Cell Image Library Group ID: 10176

• Macropus sp. MG002

• Kidney cell
• Nuclear chromosome

• Chromosome organization
• DNA metabolic process
• DNA replication involved in S phase
• PtK1

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39235

Doi: https://doi.org/10.6075/J0GF0S51

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39235

Reference

• Ma, H et al. 1998 Spatial and temporal dynamics of DNA replication sites in mammalian cells. J Cell Biol 143:1415-1425 https://www.ncbi.nlm.nih.gov/pubmed/?term=9852140

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2022-08-12